Utilizing directed evolution to interrogate and optimize CRISPR/Cas guide RNA scaffolds
Korie B. Bush, Giulia I. Corsi, Amy Yan, Keith Haynes, Juliana M. Layzer, Jonathan H. Zhou, Telmo Llanga, Jan Gorodkin, Bruce A. Sullenger
Topics & Concepts
CRISPRCas9Systematic evolution of ligands by exponential enrichmentComputational biologyGenome editingGuide RNADNABiologySubgenomic mRNARNAGeneticsGeneCRISPR and Genetic EngineeringRNA and protein synthesis mechanismsRNA regulation and disease