Ground-State Destabilization by Active-Site Hydrophobicity Controls the Selectivity of a Cofactor-Free Decarboxylase
Michal Biler, Rory Crean, Anna Katharina Schweiger, Robert Kourist, Shina Caroline Lynn Kamerlin
Abstract
) carboxylate binds. Our results allow insight into the tight interaction network determining AMDase selectivity, which in turn provides guidance for the identification of target residues for future enzyme engineering.
Topics & Concepts
ChemistryCarboxylateActive siteSelectivitySubstrate (aquarium)CofactorStereochemistryEnzymeCombinatorial chemistryBiochemistryCatalysisGeologyOceanographyBiochemical and biochemical processesEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and Bioproduction