Transfection of hard-to-transfect primary human macrophages with<i>Bax</i>siRNA to reverse Resveratrol-induced apoptosis
Simon Xin Min Dong, Ramon Edwin Caballero, Hamza Ali, David Roy, Edana Cassol, Ashok Kumar
Abstract
with this technique effectively protected primary human macrophages and PMA-differentiated U937 cells against Resveratrol-induced cell death. In addition, this method inherently takes the balance between transfection rate and cytotoxicity of siRNA transfection reagents into consideration.
Topics & Concepts
TransfectionResveratrolBiologyApoptosisSmall interfering RNACell biologyRNA interferenceMolecular biologyCell cultureGeneRNAGeneticsBiochemistryRNA Interference and Gene DeliveryImmunotherapy and Immune Responsesinterferon and immune responses