Improved Sensing Performance of Amperometric Urea Biosensor by Using Platinum Nanoparticles
Seyda Korkut, Muhammet Samet Kılıç, Fatma Yetiren
Abstract
Abstract A novel Platinum nanoparticle (PtNPs) modified Poly(pyrrole‐ co ‐1‐(2‐Aminophenyl)pyrrole)/Urease film coated Au electrode was designed for amperometric detection of urea. PtNPs quantity, film density and pH were optimized and interference effect of some substances readily found in municipal wastewater and blood was investigated. The biosensor responded to urea with a measurement concentration range of 0.1 to 30 mM, a sensitivity of 31.8 μA mM −1 cm −2 , a LOD of 7.58 μM, an accuracy of 104 % and a RSD% of only 0.82. It sensed the concentration of urea in the municipal sewage water with recovery of 97.6 % (n=3) and remained 78 % of its initial response at 28 th day. Results confirmed that PtNPs with strong conductivity improved the electron transfer ability of the working electrode.