Direct observation of surface charge and stiffness of human metaphase chromosomes
Seokbeom Roh, Taeha Lee, Da Yeon Cheong, Yeonjin Kim, Soohwan Oh, Gyudo Lee
Abstract
36.87 ± 17.56 MPa). The results suggested undulating but compact coordination of condensed chromosomes. Additionally, excessive treatment with RNase and protease could destroy the chromosomal structure, providing an exceptional opportunity for multiscale stiffness mapping of polynucleotides, nucleosomes, chromatin fibers, and chromosomes in a single image. Our approach offers a new horizon in terms of an analytical technique for studying chromosome-related diseases.
Topics & Concepts
MetaphaseChromatinChromosomeBiophysicsPolynucleotideDNAStiffnessAtomic force microscopyChemistryMaterials scienceNanotechnologyBiologyGeneticsComposite materialGeneAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery