Comparing the Potential of Silicon Nanoparticles and Conventional Silicon for Salinity Stress Alleviation in Soybean (<i>Glycine max</i> L.): Growth and Physiological Traits and Rhizosphere/Endophytic Bacterial Communities
Pan Wang, Hui Zhang, Xiao Hu, Lingling Xu, Xin An, Ting Jin, Ruixue Ma, Zhe-Fei Li, Sanfeng Chen, Sen Du, Gehong Wei, Chun Chen
Abstract
In this study, 20-day-old soybean plants were watered with 100 mL of 100 mM NaCl solution and sprayed with silica nanoparticles (SiO 2 NPs) or potassium silicate every 3 days over 15 days, with a final dosage of 12 mg of SiO 2 per plant. We assessed the alterations in the plant’s growth and physiological traits, and the responses of bacterial microbiome within the leaf endosphere, rhizosphere, and root endosphere. The result showed that the type of silicon did not significantly impact most of the plant parameters. However, the bacterial communities within the leaf and root endospheres had a stronger response to SiO 2 NPs treatment, showing enrichment of 24 and 13 microbial taxa, respectively, compared with the silicate treatment, which led to the enrichment of 9 and 8 taxonomic taxa, respectively. The rhizosphere bacterial communities were less sensitive to SiO 2 NPs, enriching only 2 microbial clades, compared to the 8 clades enriched by silicate treatment. Furthermore, SiO 2 NPs treatment enriched beneficial genera, such as Pseudomonas, Bacillus, and Variovorax in the leaf and root endosphere, likely enhancing plant growth and salinity stress resistance. These findings highlight the potential of SiO 2 NPs for foliar application in sustainable farming by enhancing plant-microbe interactions to improve salinity tolerance.