A novel fluorogenic probe for visualizing the hydrogen peroxide in Parkinson’s disease models
Gaobin Zhang, Zheng Li, Fangjie Chen, Duoteng Zhang, Wenhui Ji, Zhengpeng Yang, Qiong Wu, Chengwu Zhang, Lin Li, Wei Huang
Abstract
Parkinson’s disease (PD) is closely related to the oxidative stress induced by excess hydrogen peroxide (H 2 O 2 ) in organisms. Developing an efficient method for noninvasive and real-time H 2 O 2 detection is beneficial to investigate the role played by H 2 O 2 in PD. In this work, a novel fluorogenic probe (CBH) for living organisms H 2 O 2 detection has been designed, synthesized and characterized. The emission of CBH in PBS solution is very weak. However, when H 2 O 2 was added, the fluorescence of CBH solution was sharply increased for 12-fold, accompanied by the emission peak blue-shifted from 600 to 530 nm. Moreover, the response of CBH to H 2 O 2 is highly sensitive and selective and is not affected by various ROS/RNS, anions, cations, and amino acids. Based on the good performance of CBH for H 2 O 2 detection, it has been successfully applied to visualizing the H 2 O 2 concentration in living cells, Zebrafish and [Formula: see text]. elegans PD models.