Design, Spectral Characteristics, and Possibilities for Practical Application of BODIPY FL-Labeled Monoterpenoid
Г. Б. Гусева, Е. В. Антина, М. Б. Березин, Roman S. Pavelyev, Airat R. Kayumov, Olga V. Ostolopovskaya, Ilmir R. Gilfanov, Л. Л. Фролова, A. V. Kutchin, R. F. Akhverdiev, Svetlana А. Lisovskaya, Elena Y. Trizna, О.А. Lodochnikova, Daut R. Islamov, Sergey V. Efimov, Vladimir V. Klochkov, Ilya A. Khodov, Sergei Boichuk, Л. Е. Никитина
Abstract
)-6″,6″-dimethylbicyclo[3.1.1]hept-2″-ene-2″)yl-methoxycarbonylpropyl)-3,3',5,5'-tetramethyl-2,2'-dipyrromethene conjugate (BODIPYmyrt). The fluorophore was characterized using X-ray, NMR, MS, and UV/vis spectroscopy. The conjugate exhibits a high quantum yield (to ∼100%) in the region 515-518 nm. BODIPYmyrt effectively penetrates the membranes of the bacterial and fungal cells and therefore can be used to examine the features of a broad spectrum of Gram-positive and Gram-negative bacteria and pathogenic fungi as well. Moreover, BODIPYmyrt exhibits a moderate tropism to the subcellular structures in mammalian cells (e.g., mitochondria), thereby providing an attractive scaffold for fluorophores to examine these particular organelles.