Far-red switching DNA probes for live cell nanoscopy
Jonas Bucevičius, Tanja Gilat, Gražvydas Lukinavičius
Abstract
Herein we present DNA probes composed of Hoechst 33258 and spontaneously blinking far-red hydroxymethyl silicon-rhodamine (HMSiR). The best performing probe, 5-HMSiR-Hoechst, contains the 5'-regioisomer, shows ∼400-fold fluorescence increase upon DNA binding and is compatible with wash-free single molecule localization and 3D stimulated emission depletion microscopy of chromatin nanostructures in living cells.
Topics & Concepts
ChromatinHydroxymethylDNARhodamineChemistryBiophysicsNanotechnologyFluorescenceMaterials scienceBiologyOpticsBiochemistryPhysicsStereochemistryAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA Interference and Gene Delivery