Terbium(III) as a Fluorescent Probe for Molecular Detection of Ascorbic Acid
Н. М. Селиванова, Yu. G. Galyametdinov
Abstract
Fluorescence analysis is a simple and a highly sensitive method for detection of small amounts of biologically active substances. In this study, a complexation of terbium(III) chelates with 1,10-phenanthroline and ascorbic acid (AA) and luminescent properties of complexes were investigated. The influence of pH and solubilization of complexes by micellar solutions of nonionic, cationic, and anionic surfactants on fluorescence was studied. The quenching effect of terbium ion fluorescence was detected upon an introduction of ascorbic acid. The quenching effect of the complex with mixed ligands Tb(1,10-phenanthroline)-AA allows for the detection of ascorbic acid with the limit of 7.4 × 10−5 mol·L−1.
Topics & Concepts
Ascorbic acidTerbiumChemistryFluorescenceQuenching (fluorescence)Detection limitCationic polymerizationLuminescenceChelationInorganic chemistryPhenanthrolineNuclear chemistryPhotochemistryIonOrganic chemistryChromatographyMaterials scienceOptoelectronicsQuantum mechanicsPhysicsFood scienceLanthanide and Transition Metal ComplexesMolecular Sensors and Ion DetectionAnalytical chemistry methods development