Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells
Keiko Sakamoto, Shubham Goel, A Funakoshi, Tetsuya Honda, Keisuke Nagao
Abstract
Skin is our body's outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021).
Topics & Concepts
Flow cytometryImmune systemCellImmunologyCell biologyCytometryBiologyGeneticsDermatology and Skin DiseasesSkin Protection and AgingHair Growth and Disorders