A constitutively monomeric UVR8 photoreceptor confers enhanced UV-B photomorphogenesis
Roman Podolec, Kelvin Lau, Timothée B. Wagnon, Michael Hothorn, Roman Ulm
Abstract
was observed to be predominantly monomeric in vivo and, once activated by UV-B, was not efficiently inactivated. Analysis of a UVR8 crystal structure containing the G101S mutation revealed the distortion of a loop region normally involved in stabilization of the UVR8 homodimer. Plants expressing a UVR8 variant combining G101S with the previously described W285A mutation exhibited robust constitutive photomorphogenesis. This work provides further insight into UVR8 activation and inactivation mechanisms and describes a genetic tool for the manipulation of photomorphogenic responses.
Topics & Concepts
PhotomorphogenesisCell biologyChemistryBiologyBiochemistryArabidopsisGeneMutantLight effects on plantsPhotoreceptor and optogenetics researchPhotochromic and Fluorescence Chemistry