<scp>m<sup>6</sup>A</scp> transferase <scp>KIAA1429</scp>‐stabilized <scp>LINC00958</scp> accelerates gastric cancer aerobic glycolysis through targeting <scp>GLUT1</scp>
Desheng Yang, Shuang Chang, Fuchun Li, Mingxiao Ma, Jiayao Yang, Xun Lv, Luxin Huangfu, Changhe Jia
Abstract
Abstract Emerging evidence has demonstrated that N 6 ‐methyladenosine (m 6 A) and long noncoding RNAs (lncRNAs) are both crucial regulators in gastric cancer (GC) tumorigenesis. However, the interaction of m 6 A and lncRNAs in GC progression are still unclear. Here, our team discovered that lncRNA LINC00958 expression up‐regulated in GC tissue and cells. Clinically, high‐expression of LINC00958 was clinically correlated to lower survival of GC patients. Functionally, in vitro assays demonstrated that LINC00958 promoted the GC cells' aerobic glycolysis. Mechanistically, methylated RNA immunoprecipitation sequencing (MeRIP‐Seq) found that there were m 6 A‐modificated sites in LINC00958, and moreover m 6 A methyltransferase KIAA1429 catalyzed the m 6 A modification on LINC00958 loci. Moreover, LINC00958 interacted with GLUT1 mRNA via the m 6 A‐dependent manner to enhance GLUT1 mRNA transcript stability, thereby positively regulating the aerobic glycolysis of GC. In conclusion, our findings reveal the function and mechanism of KIAA1429‐induced LINC00958 in GC, delineating novel understanding of m 6 A‐lncRNA in cancer biology.