Full-color light-field microscopy via single-pixel imaging
Manhong Yao, Zixin Cai, Xue Qiu, Shiping Li, Junzheng Peng, Jingang Zhong
Abstract
Light-field microscopy is a scanless volumetric imaging technique. Conventional color light microscope employs a micro-lens array at the image plane and samples the spatial, angular, and color information by a pixelated two-dimensional (2D) sensor (such as CCD). However, the space bandwidth product of the pixelated 2D sensor is a fixed value determined by its parameters, leading to the trade-offs between the spatial, angular, and color resolutions. In addition, the inherent chromatic aberration of the micro-lens array also reduces the viewing quality. Here we propose full-color light-field microscopy via single-pixel imaging that can distribute the sampling tasks of the spatial, angular, and color information to both illumination and detection sides, rather than condense on the detection side. Therefore, the space bandwidth product of the light-field microscope is increased and the spatial resolution of the reconstructed light-field can be improved. In addition, the proposed method can reconstruct full-color light-field without using a micro-lens array, thereby the chromatic aberration induced by the micro-lens array is avoided. Because distributing the three sampling tasks to both the illumination and detection sides has different possible sampling schemes, we present two sampling schemes and compare their advantages and disadvantages via several experiments. Our work provides insight for developing a high-resolution full-color light-field microscope. It may find potential applications in the biomedical and material sciences.