<i>Streptomyces</i> cell-free systems for natural product discovery and engineering
Simon J. Moore, Hung‐En Lai, Jian Li, Paul S. Freemont
Abstract
Streptomyces cell-free gene expression (CFE) systems for natural product discovery. The CFE system simply requires DNA, a cell-extract, amino acids, and a metabolite solution to study transcription, translation, and biosynthesis in a single reaction.
Topics & Concepts
Natural productStreptomycesBiosynthesisComputational biologyTranscription (linguistics)GeneSecondary metaboliteDNACellBiochemistryTranslation systemChemistryBiologyCombinatorial chemistryGeneticsBacteriaPhilosophyLinguisticsIn vitroMicrobial Natural Products and BiosynthesisChemical Synthesis and AnalysisMarine Sponges and Natural Products