An endoplasmic reticulum-targeted and near-infrared fluorescent probe for determining hydrogen polysulfides derived from a naphthalimide
Yijie Ma, Junhong Xu, Qiujuan Ma, Guojiang Mao, Shuqi Hou, Yujie Lian, Ning Cui, Ruxue Xia
Abstract
Hydrogen polysulfides (H 2 S n , n > 1) was the oxidized form of H 2 S and plays significant roles in physiological and pathological processes. Abnormal levels of H 2 S n is associated with a series of physiological diseases. The endoplasmic reticulum-targetable fluorescent probes are rare for determining H 2 S n . Therefore, quantitative detection of H 2 S n levels in the endoplasmic reticulum is extremely crucial. In this study, a naphthalimide-based near-infrared fluorescent probe was constructed for detecting H 2 S n in the endoplasmic reticulum. 2-Fluoro-5-nitrobenzoate was chose as the H 2 S n sensing element. Before the addition of H 2 S n , the probe exhibited very weakly fluorescence at 700 nm. After adding H 2 S n , the probe demonstrated intense red fluorescence at 700 nm. The probe responded rapidly and exhibited excellent selectivity and sensitivity. And the probe displayed negligible cytotoxicity and had been successfully utilized for near-infrared imaging of H 2 S n within the endoplasmic reticulum of A549 cells. Additionally, the probe was also employed for imaging H 2 S n in zebrafish and exhibited satisfied results.