Cryo-EM reconstruction of a VPS13 fragment reveals a long groove to channel lipids between membranes
Peiqi Li, Joshua A. Lees, C. Patrick Lusk, Karin M. Reinisch
Abstract
A single particle cryo-EM reconstruction of an ∼160-kD N-terminal fragment of the lipid transport protein VPS13 reveals an ∼160-Å long channel lined with hydrophobic residues suitable for solubilizing multiple lipid fatty acid moieties. The structure suggests that VPS13 and related proteins, like the autophagy protein ATG2, can act as bridges between organelle membranes to allow bulk lipid flow between organelles.
Topics & Concepts
OrganelleMembraneGroove (engineering)Fragment (logic)BiophysicsChemistryParticle (ecology)BiochemistryCrystallographyBiologyMaterials scienceEcologyProgramming languageComputer scienceMetallurgyLipid metabolism and biosynthesisProtein Structure and DynamicsLipid Membrane Structure and Behavior