Promoter engineering improves transcription efficiency in biomolecular assays
Ji-Su Woo, Jung H. Kim, Seokjoon Kim, Ki Soo Park
Abstract
A novel T7 promoter with improved transcription efficiency has been developed. It is more suitable for diagnostic applications due to its small size and is successfully used for an RNase H activity assay with high sensitivity and selectivity.
Topics & Concepts
Transcription (linguistics)PromoterChemistryRNase PComputational biologySelectivityMolecular biologyNanotechnologyBiologyGeneBiochemistryGene expressionMaterials scienceRNACatalysisPhilosophyLinguisticsBacteriophages and microbial interactionsRNA and protein synthesis mechanismsAdvanced biosensing and bioanalysis techniques