Detection and Identification of<i>Acanthamoeba</i>and Other Nonviral Causes of Infectious Keratitis in Corneal Scrapings by Real-Time PCR and Next-Generation Sequencing-Based 16S-18S Gene Analysis
Dennis Back Holmgaard, Céline Barnadas, Seyed Hossein Mirbarati, Lee O’Brien Andersen, Henrik Nielsen, Christen Rune Stensvold
Abstract
Acanthamoeba is a free-living amoeba of extensive genetic diversity. It may cause infectious keratitis (IK), which can also be caused by bacteria, fungi, and viruses.
Topics & Concepts
AcanthamoebaBiologyAcanthamoeba keratitisKeratitisMicrobiologyAmoeba (genus)ProtozoaPolymerase chain reactionVirologyDNA sequencingGeneGeneticsLegionella and Acanthamoeba researchBacterial biofilms and quorum sensingOcular Infections and Treatments