Peptide and protein assays using customizable bio-affinity arrays combined with ambient ionization mass spectrometry
Xuemeng Zhang, Wei Wang, Richard N. Zare, Qianhao Min
Abstract
arbitrarily predefined affinity interactions including coordination chemistry, covalent bonding, and biological recognition. An integrated MS platform allows continuous interrogation. Profiling and quantitation of dysregulated phosphopeptides from small-volume (∼5 μL) serum samples has been successfully demonstrated. As a front-end device adapted to any mass spectrometer, this MS platform might hold much promise in protein/peptide analysis in point-of-care (POC) diagnostics and clinical applications.
Topics & Concepts
Mass spectrometryPeptideChemistryChromatographyProtein mass spectrometryIonizationElectrospray ionizationBiochemistryOrganic chemistryIonMass Spectrometry Techniques and ApplicationsAdvanced Proteomics Techniques and ApplicationsAdvanced Biosensing Techniques and Applications