Studying Gene Expression in Irradiated Barley Cultivars: PM19L-like and CML31-like Expression as Possible Determinants of Radiation Hormesis Effect
Irina Gorbatova, Elizaveta Kazakova, Mikhail Podlutskii, Ivan Pishenin, Vladimir S. Bondarenko, А. А. Донцова, Д. П. Донцов, А.С. Снегирев, Е. С. Макаренко, Sofia Bitarishvili, Maria A. Lychenkova, Т. V. Chizh, Polina Volkova
Abstract
Gamma (γ)-irradiation of plants at low doses can provoke a broad range of growth-stimulating effects. In order to reveal universal target genes that are involved in molecular pathways of radiation hormesis establishment, we studied nine barley cultivars for their tolerance to γ-irradiation of seeds. Four morphological traits were assessed in barley seedlings after γ-irradiation of seeds at 20 Gy. Nine cultivars were sorted according to the sensitivity to irradiation as γ-stimulated, “no morphological effect”, or γ-inhibited. Gene expression of 17 candidate genes was evaluated for the 7 most contrasting cultivars. Changes in expression of barley homologues of PM19L and CML31 were suggested as possible determinants of radiation hormesis effect. The possible role of jasmonate signaling in roots in radiation growth stimulations was revealed. Morphological analysis and gene expression study showed that the genetic background of a cultivar plays an important role in eustress responses to low-dose γ-irradiation of seeds.