Identification of Histone Peptide Binding Specificity and Small-Molecule Ligands for the TRIM33α and TRIM33β Bromodomains
Angelina R. Sekirnik, Jessica K. Reynolds, Larissa See, Joseph P. Bluck, Amy R. Scorah, C. Tallant, Bernadette Lee, Katarzyna B. Leszczyńska, Rachel L. Grimley, Richard Storer, Marta Malattia, Sara Crespillo, Sofia Caria, Stéphanie Duclos, Ester M. Hammond, Stefan Knapp, Garrett M. Morris, Fernanda Duarte, Philip C. Biggin, Stuart J. Conway
Abstract
K18Ac. We used this information to develop an AlphaScreen assay, with which we have identified four new ligands for the TRIM33 PHD-BRD cassette. These findings provide fundamental new information regarding which histone marks are recognized by both isoforms of TRIM33 and suggest starting points for the development of chemical probes to investigate the cellular function of TRIM33.
Topics & Concepts
BromodomainHistonePeptideSmall moleculeBiologyComputational biologyHistone H4ChemistryBiochemistryGeneProtein Degradation and InhibitorsUbiquitin and proteasome pathwaysinterferon and immune responses