Fast reconstruction algorithm for structured illumination microscopy
Shijie Tu, Qiulan Liu, Xin Liu, Wenjie Liu, Zhimin Zhang, Taojin Luo, Cuifang Kuang, Xu Liu, Xiang Hao
Abstract
Structured illumination microscopy (SIM) is a powerful technique for providing super-resolution imaging, but its reconstruction algorithm, i.e., linear reconstruction structured illumination microscopy (LRSIM) algorithm in the Fourier domain, limits the imaging speed due to its computational effort. Here, we present a novel reconstruction algorithm that can directly process SIM data in the spatial domain. Compared to LRSIM, this approach uses the same number of frames to achieve a comparable resolution but with a much faster processing speed. Our algorithm was verified on both simulated and experimental data using sinusoidal pattern illumination. Moreover, this algorithm is also applicable for speckle pattern illumination.