Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting
José Ángel Nicolás-Avila, María Sánchez-Díaz, Andrés Hidalgo
Abstract
Cardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Nicolás-Ávila et al. (2020).
Topics & Concepts
Cell sortingCell biologySortingFluorescenceCellIsolation (microbiology)ExtracellularChemistryExtracellular vesiclesBiologyMolecular biologyBiochemistryComputer scienceBioinformaticsPhysicsQuantum mechanicsProgramming languageExtracellular vesicles in diseaseTissue Engineering and Regenerative MedicineCardiac Fibrosis and Remodeling