Calixarene-modified albumin for stoichiometric delivery of multiple drugs in combination-chemotherapy
Ying Wang, Zhanzhan Zhang, Xinzhi Zhao, Lina Xu, Yadan Zheng, Huabin Li, Dong‐Sheng Guo, Linqi Shi, Yang Liu
Abstract
In combination chemotherapy, the molar ratio of drugs is a critical parameter that determines the synergistic effects. However, most co-delivery vectors are incapable of maintaining the optimal molar ratio of drugs throughout the delivery process. Herein, a calixarene-modified albumin (CaMA), which can co-deliver multiple drugs with precise control of the drug ratio, is presented. Methods: CaMA was prepared by chemically conjugating multiple sulfonate azocalix[4]arenes (SAC4A) onto the surface of bovine serum albumin (BSA). The precise drug loading and synchronous drug release were measured using fluorescence spectroscopy. Mouse tumor cell 4T1 and 4T1-bearing mice were used to evaluate the combined effects of mitomycin C (MMC) and doxorubicin (DOX) in vitro and in vivo.