Antiviral and anti-inflammatory activities of chemical constituents from twigs of Mosla chinensis Maxim
Shi-yan Feng, Na Jiang, Jiaying Yang, Linyao Yang, Jiangchao Du, Xuan‐Qin Chen, Dan Liu, Rong‐Tao Li, Jin-Dong Zhong
Abstract
Abstract Seven undescribed compounds, including three flavones ( 1–3 ), one phenylpropanoid ( 19 ), three monoaromatic hydrocarbons ( 27–29 ), were isolated from the twigs of Mosla chinensis Maxim together with twenty-eight known compounds. The structures were characterized by HRESIMS, 1D and 2D NMR, and ECD spectroscopic techniques. Compound 20 displayed the most significant activity against A/WSN/33/2009 (H1N1) virus (IC 50 = 20.47 μ M) compared to the positive control oseltamivir (IC 50 = 6.85 µ M). Further research on the anti-influenza mechanism showed that compound 20 could bind to H1N1 virus surface antigen HA1 and inhibit the early attachment stage of the virus. Furthermore, compounds 9 , 22 , 23 , and 25 displayed moderate inhibitory effects on the NO expression in LPS inducing Raw 264.7 cells with IC 50 values of 22.78, 20.47, 27.66, and 30.14 µ M, respectively. Graphical Abstract