Engineering D-allulose 3-epimerase from Clostridium cellulolyticum for improved thermostability using directed evolution facilitated by a nonenzymatic colorimetric screening assay
Jiajun Chen, Zhaolin Huang, Ting Shi, Dawei Ni, Yingying Zhu, Wei Xu, Wenli Zhang, Wanmeng Mu
Topics & Concepts
ThermostabilityMutantDirected evolutionMutagenesisProtein engineeringEnzymeChemistryHigh-throughput screeningYield (engineering)Wild typeBiochemistryActive siteGeneMaterials scienceMetallurgyDiet, Metabolism, and DiseasePancreatic function and diabetesPolyamine Metabolism and Applications