Molecular Cloning, Characterization, and Application of Organic Solvent-Stable and Detergent-Compatible Thermostable Alkaline Protease from <i>Geobacillus thermoglucosidasius</i> SKF4
Suleiman Dan Allison, Nur AdeelaYasid, Fairolniza Mohd Shariff, Nor’Aini Abdul Rahman
Abstract
concentration. The activity of the protease was completely inhibited (100%) by phenylmethylsulfonyl fluoride (PMSF); however, the addition of sodium dodecyl sulfate (SDS) resulted in a 20% increase in activity. The enzyme was also stable in various organic solvents and in certain commercial detergents. Furthermore, the enzyme exhibited strong potential for industrial use, particularly as a detergent additive and for facilitating the recovery of silver from X-ray film.
Topics & Concepts
ProteasesCloning (programming)Alkaline proteaseProteaseThermophileSerine proteaseMolecular cloningclone (Java method)BiochemistryBiologyChemistryGeneEnzymePeptide sequenceProgramming languageComputer scienceEnzyme Production and CharacterizationBiofuel production and bioconversionEnzyme Structure and Function