Tips for improving genome annotation quality

Abstract

With the advancement of sequencing technology and genome assembly algorithms, we can easily obtain high-quality genome assembly results, however, the remaining challenge is accurate genome annotation. As the cornerstone for downstream analysis, there are many details during the genome annotation process that are worth discussing or mentioning. To reduce the annotation errors, we briefly list six tips during the annotation process, and the noteworthy details in different widely used annotation tools/pipelines such as MAKER and EvidenceModeler, to improve annotation quality.

Topics & Concepts