The wound healing effect of collagen/adipose‐derived stem cells (ADSCs) hydrogel: In vivo study
Kamyar Abbasi, Sara Tavakolizadeh, Alireza Hadi, Maryam Hosseini, Reza Sayyad Soufdoost, Artak Heboyan, Mostafa Alam, Sadaf Fani‐Hanifeh
Abstract
BACKGROUND: The complex wound healing process involves activating and synchronizing intracellular, intercellular, and extracellular components. Adipose tissue is attracting attention to promote wound healing. Within subcutaneous adipose tissue, stromal vascular cells and their subsets release growth factors and cytokines critical for neovascularization and wound repair. OBJECTIVES: This study evaluated human placental collagen/adipose-derived stem cells (ADSCs) hydrogel for wound healing in rats. METHODS: In this study, ADSCs were harvested, cultured, and mixed with placental collagen. Twelve rats were used, and their backs were excised three times each. Group one received collagen/ADSCs, group two collagen, and group three non-filled (control) excisions. The healing processes were assessed by histological analysis, taking photographs, and calculating the percentage of wound contraction in mentioned times. RESULTS: Histopathological analysis revealed that the content of fibroblasts, follicles of the hair, and angiogenesis in group one was significantly more than in other groups. Group one had a significant result compared with the collagen and control groups. In group one, significant wound healing and wound contraction were observed with 52% and 80% wound contraction at 7 and 14 days, respectively. CONCLUSION: Collagen/ADSCs can be considered a suitable candidate hydrogel in wound healing with a high potential for enhancing wound repairing.