Protocol for Isolation of Viable Adult Rat Cardiomyocytes with High Yield
Xiangang Tian, Meng Gao, Anqi Li, Bilin Liu, Wenting Jiang, Yuan Qin, Guohua Gong
Abstract
Isolation of high-quantity and high-quality ventricular cardiomyocytes from adult rats is critical to study heart physiology and pathology and for drug toxicity screening. It remains challenging to produce a high yield of viable cardiomyocytes from rats. Here, we present our modified enzymatic digestion protocol that relies on the Langendorff device to generate large numbers of viable cardiomyocytes consistently. The most critical parts of this protocol are the selection of rat age and digestion time to obtain viable cardiomyocytes. For complete details on the use and execution of this protocol, please refer to Liu et al. (2019) and Qin et al. (2020).
Topics & Concepts
Protocol (science)Isolation (microbiology)Yield (engineering)Selection (genetic algorithm)ToxicityMedicineComputer scienceComputational biologyPharmacologyBiologyBioinformaticsInternal medicinePathologyMachine learningMaterials scienceMetallurgyAlternative medicineCardiac electrophysiology and arrhythmiasNeuroscience and Neural EngineeringFuel Cells and Related Materials