Spectrophotometric Determination of tetracycline hydrochloride Using 2,4 –dinitrophenyl hydrazine as Coupling Reagent
Roaa M. Khaleel, Dawooh H. Mohammed
Abstract
Abstract Simple, highly sensitive and accurate spectrophotometric method has been developed to determination of tetracycline hydrochloride in aqueous solution. The proposed method is based on the coupling of tetracycline hydrochloride with 2,4-dinitrophenylhydrazine (2,4-DNPH) in the presence of potassium periodate to form an intense orange color dye at 360nm. Beer’s law is obeyed in the concentration range 0.1-9 μg/ml with the molar absorptivity of 1.262 ×10 4 liter.mol −1 .cm −1 , with limit of detection (LOD) of 0.0123μg/ml and limit of quantification (LOQ) of 0.0412μg/ml while the RSD value of 0.184 – 0.467 % depending on the concentration. The proposed method was performed successfully to the determination and analysis of tetracycline hydrochloride in pharmaceutical formulations with average recovery of 100.23 %.