Imaging flow cytometry challenges the usefulness of classically used extracellular vesicle labeling dyes and qualifies the novel dye Exoria for the labeling of mesenchymal stromal cell–extracellular vesicle preparations
Tobias Tertel, Melanie Schoppet, Oumaima Stambouli, Ali Al-Jipouri, Patrick F. James, Bernd Giebel
Topics & Concepts
Flow cytometryMesenchymal stem cellExtracellular vesicleCalceinExtracellular vesiclesStromal cellCell biologyChemistryMicrovesiclesLive cell imagingIntracellularCellExtracellularFluorescent labellingBiophysicsFluorescenceBiologyBiochemistryMolecular biologyMembraneCancer researchPhysicsGeneQuantum mechanicsmicroRNAExtracellular vesicles in diseaseImmune cells in cancerNeutrophil, Myeloperoxidase and Oxidative Mechanisms