AAV genome modification for efficient AAV production
Walaa Asaad, Polina Volos, Denis Maksimov, Elena Khavina, Andrei A. Deviatkin, Olga Mityaeva, Pavel Volchkov
Abstract
The adeno-associated virus (AAV) is one of the most potent vectors in gene therapy. The experimental profile of this vector shows its efficiency and accepted safety, which explains its increased usage by scientists for the research and treatment of a wide range of diseases. These studies require using functional, pure, and high titers of vector particles. In fact, the current knowledge of AAV structure and genome helps improve the scalable production of AAV vectors. In this review, we summarize the latest studies on the optimization of scalable AAV production through modifying the AAV genome or biological processes inside the cell.
Topics & Concepts
GenomeAdeno-associated virusVector (molecular biology)Genetic enhancementComputational biologyBiologyVirologyComputer scienceGeneGeneticsRecombinant DNAVirus-based gene therapy researchViral Infectious Diseases and Gene Expression in InsectsRNA Interference and Gene Delivery