Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification
Shiwen Wu, Wen‐Han Yu, Xian‐Shu Fu, Xiaoping Yu, Zihong Ye, Mingzhou Zhang, Yulou Qiu, Biao Ma
Abstract
Recombinase polymerase amplification (RPA) has emerged as a rapid, efficient, and highly sensitive method for nucleic acid amplification, thus becoming a focal point of research in the field of virus detection. This paper provides an overview of RPA, emphasizing its unique double-stranded DNA synthesis mechanism, rapid amplification efficiency, and capability to operate at room temperature, among other advantages. In addition, strategies and case studies of RPA in combination with other technologies are detailed to explore the advantages and potential of these integrated approaches for virus detection. Finally, the development prospect of RPA technology is prospected.