Peroxiredoxin 1 Interacts with TBK1/IKKε and Negatively Regulates Pseudorabies Virus Propagation by Promoting Innate Immunity
Lin Lv, Juan Bai, Yanni Gao, Ling Jin, Xianwei Wang, Mingzhu Cao, Xing Liu, Ping Jiang
Abstract
PRV interacts with numerous cellular proteins during productive infection. Here, we demonstrated the interaction of viral protein UL13 with the antioxidant enzyme PRDX1, which functions in multiple signal transduction pathways. We found that PRDX1 participates in the type I IFN pathway by interacting with TBK1 and IKKε, thereby negatively regulating PRV propagation. However, UL13 ubiquitinates PRDX1, which routes PRDX1 into proteasomes for degradation and effectively reduces its expression. These results illuminate the fundamental role that PRDX1 plays in the IFN pathway, and they identify a potential target for the control of PRV infection.
Topics & Concepts
BiologyTANK-binding kinase 1Innate immune systemCell biologyIκB kinaseTRIFSignal transductionKinaseProteasomeAutophagyNF-κBImmune systemProtein kinase AMAP kinase kinase kinaseImmunologyGeneticsToll-like receptorApoptosisMosquito-borne diseases and controlViral Infections and Vectorsinterferon and immune responses