SERS‐based immunomagnetic bead for rapid detection of H5N1 influenza virus
Xiwen Wang, Song Li, Han Qu, Liangyu Hao, Ting Shao, Kai Wang, Zhiping Xia, Zhiping Li, Qianxue Li
Abstract
Abstract The surface‐enhanced Raman scattering (SERS) has recently drawn attention in the detection of respiratory viruses, but there have been few reports of the direct detection of viruses. In this study, a sandwich immunomagnetic bead SERS was established for the rapid diagnosis of the H5N1 influenza virus. The detection limit was estimated to be 5.0 × 10 −6 TCID 50 /ml. The method showed excellent specificity with no cross‐reaction with H1N1, H5N6 or H9N2. The H5N1 influenza virus detection accuracy of the SERS method was 100% in chicken embryos. The results hold great promise for the utilization of SERS as an innovative approach in the diagnosis of influenza virus.