Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis
Thomas Haas, Danye Qiu, Markus Häner, Larissa Angebauer, Alexander Ripp, Jyoti Singh, Hans‐Georg Koch, Claudia Jessen‐Trefzer, Henning J. Jessen
Abstract
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.
Topics & Concepts
Capillary electrophoresisNucleotideReagentChemistryPhosphoramiditeMAGIC (telescope)ElectrophoresisCapillary actionChromatographyBiochemistryDNAMaterials scienceOrganic chemistryPhysicsGeneOligonucleotideQuantum mechanicsComposite materialRNA and protein synthesis mechanismsBacteriophages and microbial interactionsAdvanced biosensing and bioanalysis techniques