Using Fiber Photometry in Mice to Estimate Fluorescent Biosensor Levels During Sleep
Mie Andersen, Anastasia Tsopanidou, Tessa Radovanovic, Viviane Noelani Compere, Natalie Hauglund, Maiken Nedergaard, Celia Kjærby
Abstract
Sleep is not homogenous but contains a highly diverse microstructural composition influenced by neuromodulators. Prior methods used to measure neuromodulator levels in vivo have been limited by low time resolution or technical difficulties in achieving recordings in a freely moving setting, which is essential for natural sleep. In this protocol, we demonstrate the combination of electroencephalographic (EEG)/electromyographic (EMG) recordings with fiber photometric measurements of fluorescent biosensors for neuromodulators in freely moving mice. This allows for real-time assessment of extracellular neuromodulator levels during distinct phases of sleep with a high temporal resolution.