Eliminating host-guest incompatibility via enzyme mining enables the high-temperature production of N-acetylglucosamine
Yutong Wu, Jiongqin Liu, Xiao Han, Xuanlin Meng, Mengke Li, Jing Wang, Hongsong Xue, Yuhan Yang, Ping Xu, Fei Tao
Abstract
-acetylglucosamine (GlcNAc) production with high titers of up to 119.3 g/L, with the aid of systems metabolic engineering and temperature programming. This study demonstrates the effectiveness of the enzyme mining strategy, highlighting the application prospects of mining new enzymes from massive databases and providing an effective solution for tackling host-guest incompatibility.
Topics & Concepts
Host (biology)EnzymeThermophileComputational biologyThermostabilityChassisComputer scienceBiochemical engineeringChemistryBiologyBiochemistryEngineeringGeneticsStructural engineeringCarbohydrate Chemistry and SynthesisEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and Bioproduction