L-bodies are RNA–protein condensates driving RNA localization in <i>Xenopus</i> oocytes
Christopher R. Neil, Samantha P. Jeschonek, Sarah E. Cabral, Liam C. O’Connell, Erin A. Powrie, Jessica P. Otis, Timothy R. Wood, Kimberly L. Mowry
Abstract
In Xenopus oocytes, RNAs required for proper germ layer patterning localize to the vegetal cytoplasm in RNP granules, which we have termed localization bodies (L-bodies). In this work, we find that L-bodies are large, biomolecular condensates composed of a nondynamic mRNA-containing substructure enmeshed in a dynamic protein-containing layer.
Topics & Concepts
RibonucleoproteinBiologyCell biologyRNARNA-binding proteinCytoplasmRibonucleoprotein particleTranslation (biology)Rna processingFunction (biology)Subcellular localizationMessenger RNPStress granuleGranule (geology)Messenger RNATranscription (linguistics)RNA silencingSmall nucleolar RNAP-bodiesEmbryonic stem cellNucleic acid structureRibosomeBiophysicsProtein biosynthesisRNA Research and SplicingNuclear Structure and FunctionPlant Reproductive Biology