Probiotic and Functional Properties of Limosilactobacillus reuteri INIA P572
Patricia Diez‐Echave, Izaskun Martín‐Cabrejas, José Garrido‐Mesa, Susana Langa, Teresa Vezza, José María Landete, Laura Hidalgo‐Garcia, Francesca Algieri, Melinda J. Mayer, Arjan Narbad, Ana García–Lafuente, Margarita Médina, Alba Rodríguez‐Nogales, Maria Elena Rodríguez‐Cabezas, Júlio Gálvez, Juan Luís Arqués
Abstract
INIA P572 is a strain able to produce the antimicrobial compound reuterin in dairy products, exhibiting a protective effect against some food-borne pathogens. In this study, we investigated some probiotic properties of this strain such as resistance to gastrointestinal passage or to colonic conditions, reuterin production in a colonic environment, and immunomodulatory activity, using different in vitro and in vivo models. The results showed a high resistance of this strain to gastrointestinal conditions, as well as capacity to grow and produce reuterin in a human colonic model. Although the in vitro assays using the RAW 264.7 macrophage cell line did not demonstrate direct immunomodulatory properties, the in vivo assays using a Dextran Sulphate Sodium (DSS)-induced colitic mice model showed clear immunomodulatory and protective effects of this strain.