ACE2-Independent Bat Sarbecovirus Entry and Replication in Human and Bat Cells
Hua Guo, Ang Li, Tian-Yi Dong, Jia Su, Yulin Yao, Yan Zhu, Zheng‐Li Shi, Michael Letko
Abstract
Our studies demonstrate that some unexplored sarbecoviruses are capable of replicating in human and bat cells in an ACE2-independent way but need a high trypsin environment. We found that trypsin is not compensated by other known proteases involved in some coronavirus entry. This work provides important information that the trypsin-dependent entry may be a widely employed mechanism for coronaviruses and will help for further understanding the biological features of the less-studied viruses.
Topics & Concepts
FurinBiologyVirologyViral replicationViral entryProteaseTrypsinVirusSubgenusCell cultureRecombinant DNAIn vitroCell biologyEnzymeGeneGeneticsZoologyBiochemistryGenusSARS-CoV-2 and COVID-19 ResearchAnimal Virus Infections StudiesVirus-based gene therapy research