A diketopyrrolopyrrole-based ratiometric fluorescent probe for endogenous leucine aminopeptidase detecting and imaging with specific phototoxicity in tumor cells
Weibo Xu, Jian Wang, Chenyang Xu, Jianli Hua, Yu Wang
Abstract
, and DPP-Leu was successfully applied to detect LAP in fetal bovine serum (FBS) and artificial urine. Cell imaging experiments revealed that DPP-Leu could target mitochondria and distinguish tumor cells with high LAP content from normal cells. Importantly, benefiting from the structural transformation of DPP-Leu to the photosensitizer 4 under LAP catalysis, the probe could kill tumor cells under light irradiation without damaging normal cells.
Topics & Concepts
AminopeptidaseFluorescenceLeucineEndogenyPhototoxicityDetection limitEnzymeBiochemistryMolecular biologyChemistryBiophysicsBiologyIn vitroAmino acidChromatographyPhysicsQuantum mechanicsNanoplatforms for cancer theranosticsPeptidase Inhibition and AnalysisAdvanced biosensing and bioanalysis techniques