Melting dsDNA Donor Molecules Greatly Improves Precision Genome Editing in <i>Caenorhabditis elegans</i>
Krishna S. Ghanta, Craig C. Mello
Abstract
Abstract Melting and fast cooling double stranded DNA donor molecules prior to injection dramatically increases the frequency of homology-directed repair for edits such as insertions of fluorescent protein markers in Caenorhabditis elegans. Strategies described here enable consistently .....
Topics & Concepts
Caenorhabditis elegansBiologyGenome editingGenomeGeneticsComputational biologyGeneCRISPR and Genetic EngineeringGenetics, Aging, and Longevity in Model OrganismsEvolution and Genetic Dynamics