Phyto-fabrication of biocompatible silver nanoparticles using Potentilla chinensis Ser leaves: characterization and evaluation of its antibacterial activity
Kumar Vishven Naveen, Hye‐Young Kim, Kandasamy Saravanakumar, Arokia Vijaya Anand Mariadoss, Myeong‐Hyeon Wang
Abstract
The present study designed to fabricate the silver nanoparticles from the aqueous leaf extracts of Potentilla chinensis Ser (Pc-AgNPs) and their physicochemical characterization were affirmed by spectroscopic and analytical techniques. The absorption maxima at 430 nm verified the synthesis of Pc-AgNPs. Further, the XRD spectroscopy authenticated the crystal, face-centered cubic (fcc) structure of Pc-AgNPs with the 2Ө value of 38.13°, 44.34°, 64.31° and 77.36°. FT-IR spectrum revealed the contribution of functional biomolecules in reduction of Ag + to Ag 0 . Further the zeta potential, particle size and polydispersity index (PDI) of Pc-AgNPs were found as -36.6 ± 0.52 mV, 136 ± 24.82 nm and 0.202, respectively. Besides, the anisotropic structure of Pc-AgNPs with an average size of 24.3 ± 4.6 nm was confirmed by TEM analysis. In vitro antibacterial action of Pc-AgNPs was explored by agar well diffusion, then microtiter method were performed to detect the MIC and MBC of Pc-AgNPs. Further, the cellular changes in bacterial cells were visualized by confocal laser scanning and scanning electron microscopy. Pc-AgNPs considerably inhibited the growth of Salmonella enterica , Staphylococcus aureus , Bacillus cereus and Escherichia coli . The minimum inhibitory concentration (MIC) of Pc-AgNPs was found for S. aureus (5.0 ± 1.15 µg/mL), B. cereus (6.25 ± 0.58 µg/mL), S. enterica (3.75 ± 0.73 µg/mL) and E. coli (3.12 ± 1.25 µg/mL). Besides the biocompatible nature of Pc-AgNPs was explored in HEK-293 cell line, which revealed that up to 500 µg/mL did not cause any significant toxicity. Taken together, our results conclude that Pc-AgNPs have the potential antibacterial activity with promising biocompatibility in HEK-293 cells. Graphic abstract