High-throughput optofluidic screening for improved microbial cell factories <i>via</i> real-time micron-scale productivity monitoring
Matthew Rienzo, Ke-Chih Lin, Kellen C. Mobilia, Eric K. Sackmann, Volker Kurz, Adam H. Navidi, Jarett King, Robert M. Onorato, Lawrence K. Chao, Tony Wu, Hanxiao Jiang, Justin K. Valley, Troy A. Lionberger, Michael D. Leavell
Abstract
We describe high-throughput (>10<sup>3</sup> strains per week) methods for discovery of engineered microbial strains with improved secretion phenotype. These novel approaches use real-time monitoring of colony productivity under steady-state or batch culture.
Topics & Concepts
ThroughputProductivityHigh-throughput screeningScale (ratio)Biochemical engineeringNanotechnologyProcess engineeringEngineeringChemistryMaterials scienceBiochemistryPhysicsQuantum mechanicsEconomicsWirelessTelecommunicationsMacroeconomicsMicrofluidic and Bio-sensing Technologies3D Printing in Biomedical ResearchCell Image Analysis Techniques