Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification
Zhian Hu, Fujian Xu, Gongwei Sun, Sichun Zhang, Xinrong Zhang
Abstract
A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.
Topics & Concepts
Rolling circle replicationMultiplexingHomogeneousLigationmicroRNAComputer scienceMaterials scienceChemistryPhysicsMolecular biologyBiologyTelecommunicationsDNAGeneBiochemistryStatistical physicsDNA replicationAdvanced biosensing and bioanalysis techniquesBiosensors and Analytical DetectionAdvanced Fluorescence Microscopy Techniques