Directed Evolution Methods for Enzyme Engineering
Saurabh Nirantar
Abstract
Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability. A rich and growing variety of wet-lab methods have been developed by researchers over decades to accomplish this goal. In this review such methods and their specific attributes are examined.
Topics & Concepts
Biochemical engineeringVariety (cybernetics)Directed evolutionEnzymeBiotechnologyComputational biologyComputer scienceBiologyEngineeringBiochemistryArtificial intelligenceMutantGeneEnzyme Catalysis and ImmobilizationInnovative Microfluidic and Catalytic Techniques InnovationMicrobial Metabolic Engineering and Bioproduction