A field guide to Aurora kinase inhibitors: an oocyte perspective
Cecilia S. Blengini, Gyu Ik Jung, Mansour Aboelenain, Karen Schindler
Abstract
In brief: The Aurora protein kinases have critical functions in controlling oocyte meiotic maturation. In this study, we describe an assay for examining their activation state in oocytes and establish the best working doses of three commonly used inhibitors. Abstract: Several small molecule inhibitors exist for targeting Aurora kinase proteins in somatic cells. From this point of view, we evaluate the specificity of these inhibitors in mouse oocytes, and we demonstrate that MLN 8237 and AZD 1152 are specific for Aurora kinase A and Aurora kinase C, respectively, only when used at low concentrations.
Topics & Concepts
OocyteKinaseAurora A kinaseCell biologyAurora kinaseSomatic cellProtein kinase AChemistryBiologyBiochemistryEmbryoCellCell cycleGeneMicrotubule and mitosis dynamicsReproductive Biology and FertilityEpigenetics and DNA Methylation